Related Experiment Video

Updated: Jan 18, 2026

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos
07:24

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos

Published on: December 13, 2018

6.8K

Long non-coding RNA STMN1P2 promotes breast cancer doxorubicin resistance by inhibiting pyroptosis through the

You-Ping Jin1, Bu-Jie Xu1, Xiu-Fen Zhang1

  • 1Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.

Acta Pharmacologica Sinica
|September 9, 2025
PubMed

Insights

This study reveals that the long non-coding RNA STMN1P2 promotes chemoresistance in breast cancer by inhibiting pyroptosis. Reducing STMN1P2 levels can re-sensitize cancer cells to doxorubicin, offering a potential therapeutic strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Chemotherapeutic resistance, particularly to doxorubicin, is a major challenge in breast cancer treatment.
  • Pyroptosis inhibition is linked to chemoresistance, and long non-coding RNAs (lncRNAs) are implicated in cancer progression and drug resistance.

Purpose of the Study:

  • To investigate the role of the lncRNA STMN1P2 in doxorubicin resistance in breast cancer.
  • To explore the correlation between STMN1P2 and pyroptosis inhibition in breast cancer cells.

Main Methods:

  • Quantitative real-time PCR to measure STMN1P2 expression in resistant vs. sensitive tissues and cells.
  • Cell viability assays and pyroptosis marker analysis (NLRP3, ASC, caspase-1, GSDMD) after STMN1P2 knockdown or overexpression.
  • RNA immunoprecipitation and Western blotting to assess interactions between STMN1P2, hnRNPU, EZH2, TRAF6, MALT1, and caspase-1.
  • Xenograft mouse models to evaluate the in vivo effect of STMN1P2 knockdown on tumor growth suppression by doxorubicin.

Main Results:

  • STMN1P2 expression was significantly higher in doxorubicin-resistant breast cancer tissues and cells.
  • Knockdown of STMN1P2 decreased doxorubicin resistance and restored pyroptosis.
  • STMN1P2 positively regulated hnRNPU, which in turn recruited EZH2 to suppress pyroptosis pathways (TRAF6-MALT1-caspase-1).
  • Knockdown of STMN1P2 in vivo significantly enhanced doxorubicin's tumor growth suppression.

Conclusions:

  • STMN1P2 plays a critical role in promoting doxorubicin resistance in breast cancer by inhibiting pyroptosis through the hnRNPU/EZH2/TRAF6 pathway.
  • Targeting STMN1P2 presents a promising strategy for overcoming breast cancer chemoresistance.
Keywords:
breast cancerdoxorubicin resistancehnRNPUlncRNA STMN1P2pyroptosis

More Related Videos

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

26.2K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.8K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.8K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.9K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.1K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
11.7K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.6K