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A new mass spectrometry method effectively screens fentanyl analogues, identifying 250 synthetic opioids. This technique separates isomers using liquid chromatography-trapped ion mobility spectrometry-tandem mass spectrometry (LC-TIMS-q-TOF MS/MS), aiding overdose prevention.

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Area of Science:

  • Forensic Chemistry
  • Analytical Chemistry
  • Pharmacology

Background:

  • Fentanyl analogues are a major cause of overdose deaths globally.
  • Illicitly produced fentanyl is exceptionally potent and diverse.
  • Effective screening methods are crucial for public health and safety.

Purpose of the Study:

  • To develop a high-throughput mass spectrometry method for screening fentanyl analogues.
  • To achieve isomeric separation of synthetic opioids.
  • To provide a reliable analytical tool for forensic and clinical toxicology.

Main Methods:

  • Utilized liquid chromatography, trapped ion mobility spectrometry, and tandem mass spectrometry (LC-TIMS-q-TOF MS/MS).
  • Employed parallel accumulation followed by sequential fragmentation (PASEF) for enhanced sensitivity.
  • Analyzed 250 synthetic opioids based on isotopic patterns, retention times, mobility, and MS/MS fragmentation.

Main Results:

  • Successfully separated and identified 250 synthetic opioids, including isomers.
  • Observed dual mobility band profiles for most fentanyl analogues, indicating multiple protonation sites.
  • Achieved high resolution (RIMS ≈ 80-120) with low limits of detection (0.08-4 ng/mL).

Conclusions:

  • The developed LC-TIMS-q-TOF MS/MS method is effective for high-throughput screening and isomeric separation of fentanyl analogues.
  • The method maintains sensitivity while improving analytical specificity.
  • This approach offers a valuable tool for combating the synthetic opioid crisis.