Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Automated Microbial Diagnostics

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

FGF18 mediates fibroblast-leukemia crosstalk to promote acute myeloid leukemia progression.

Blood·2026

Same author

A 3D Graphene Oxide Model Reveals Fine Particulate Matter Induced Cell Cycle Dysregulation in Neural Stem Cells.

Toxics·2026

Same author

Beyond the protocol: Making pulmonary rehabilitation stick in complex COPD care.

Lung India : official organ of Indian Chest Society·2026

Same author

Investigation of the Inoculum Effect of Antimicrobial Peptides Utilizing a Microfluidic Chip Platform.

Analytical chemistry·2026

Same author

Can reusable linen truly reduce ICU emissions? Addressing key limitations - Letter on Feely et al.

Intensive & critical care nursing·2026

Same author

Deoxynivalenol drives liver injury progression by dysregulating core molecular networks: integrated multi-omics, network toxicology and molecular docking analysis.

Environment international·2026

Same journal

A two-step centrifugal microfluidic platform for semi-automated IGRA detection of tuberculosis based on chemiluminescence.

The Analyst·2026

Same journal

On-site rapid identification of animal and plant creams <i>via</i> 2D FeB nanozyme-based colorimetric sensors.

The Analyst·2026

Same journal

Sensitive detection of aflatoxin B1 using a dual-mode fluorescent aptasensor based on cascade signal amplification.

The Analyst·2026

Same journal

Deep learning-enabled microfluidic digital PCR platform for efficient seven-color quantification.

The Analyst·2026

Same journal

Monitoring food spoilage biogenic amines utilizing a blue-emitting fluorescent ionic liquid.

The Analyst·2026

Same journal

Correction: Regeneration-on-a-chip: a planarian microfluidic device enabling automated cultivation, individual tracking and <i>in vivo</i> imaging for regeneration study.

The Analyst·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 30, 2026

Microfluidic Chip Fabrication and Method to Detect Influenza

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

A directional self-priming continuous-driven compartmentalized microfluidic chip for multiplexed pathogen detection.

Weibo Fang¹, Yating Pan¹, Mariam Maiga¹

¹Research Centre for Analytical Instrumentation, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310027, P. R. China. muying@zju.edu.cn.

|September 10, 2025

Summary

This summary is machine-generated.

A novel snowflake-shaped microfluidic chip enables rapid, simultaneous detection of multiple foodborne pathogens. This easy-to-use device offers high sensitivity and efficiency, crucial for food safety and preventing food poisoning.

More Related Videos

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Related Experiment Videos

Last Updated: Jun 30, 2026

Microfluidic Chip Fabrication and Method to Detect Influenza

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Area of Science:

Biotechnology
Microfluidics
Food Safety

Background:

Multiplexed nucleic acid detection is vital for foodborne pathogen screening but faces challenges with complex designs and unreliable sample introduction.
Existing methods often lead to complicated operations, primer/probe crosstalk, and false positives, hindering efficient pathogen detection.

Purpose of the Study:

To develop an easy-to-operate, stable, and highly efficient multiplexed detection method for foodborne pathogens.
To overcome the limitations of current multiplexed nucleic acid detection techniques, including complex designs and sample introduction issues.

Main Methods:

Developed a snowflake-shaped microfluidic chip with six branches for simultaneous multi-target detection from a single sample using monochrome fluorescence.
Utilized PDMS negative pressure for a self-priming, continuous-driven method, eliminating the need for external power sources and enabling efficient reagent loading with 100% compartmentalization.
Validated the chip's performance by detecting three foodborne pathogens: Salmonella enterica, Staphylococcus aureus, and Listeria monocytogenes.

Main Results:

The microfluidic chip successfully enabled simultaneous detection of multiple foodborne pathogens.
Achieved high sensitivity, detecting targets as low as 10 copies per μL.
Demonstrated reduced reagent consumption and sample loss due to the outlet-free, compartmentalized microchannel design.

Conclusions:

The snowflake-shaped microfluidic chip provides a robust and efficient platform for multiplexed foodborne pathogen detection.
This technology shows significant potential for point-of-care (POC) applications in food safety monitoring.
The developed chip addresses key challenges in multiplexed detection, offering a promising solution for rapid screening.