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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Jan 18, 2026

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray

Published on: January 6, 2016

A barcode-specific immobilization interface for microfluidics-assisted uniform spatially barcoded microarray

Sidi Tian^1,2, Yingxue Li², Jia Yao²

¹School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China.

|September 10, 2025

Summary

This summary is machine-generated.

This study introduces a barcode-specific immobilization (BarSI) interface to create uniform spatially barcoded microarray chips. This novel method ensures unbiased transcriptome analysis for high-throughput spatial transcriptomics.

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Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

Area of Science:

Biotechnology
Genomics
Microfluidics

Background:

Spatial transcriptomic profiling is essential for understanding tissue organization.
Current microfluidics-based methods suffer from non-uniform barcoding due to reagent adsorption, leading to biased mRNA analysis.
Uniform barcoded microarrays are critical for accurate spatial transcriptomic data.

Purpose of the Study:

To develop a novel interface for uniform barcoding in microfluidic microarray chips.
To overcome the limitations of non-specific adsorption in microchannels.
To enable unbiased transcriptome capture and analysis.

Main Methods:

Development of a barcode-specific immobilization (BarSI) interface with anti-adsorption and biological activity.
Immobilization of DNA probes in microchannels, achieving low coefficients of variation (CV).
Fabrication of spatially barcoded microarray chips using an orthogonal barcoding system.

Main Results:

Achieved highly uniform DNA probe immobilization in microchannels (CV of 2.3% and 3.2%).
Developed spatially barcoded microarray chips with significantly reduced fluorescence intensity CV (8.47 ± 1.26%) compared to conventional slides (20.91 ± 2.84%).
Demonstrated spatially unbiased detection of mouse liver mRNA with Moran's I < 0.05.

Conclusions:

The BarSI interface enables the economical and accessible manufacturing of uniform spatially barcoded microarray chips.
This technology provides a novel strategy for unbiased transcriptome analysis.
The developed method significantly improves the accuracy and reliability of spatial transcriptomic profiling.