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Higher-order structure of long repeat chromatin.

J Widom, J T Finch, J O Thomas

    The EMBO Journal
    |December 1, 1985
    PubMed
    Summary

    Sea urchin sperm chromatin

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    Area of Science:

    • Chromatin higher-order structure
    • Molecular biology
    • Biophysics

    Background:

    • Chromatin higher-order structure determines DNA accessibility and gene regulation.
    • Sea urchin sperm chromatin exhibits an unusually long nucleosomal DNA repeat length (~240 bp).

    Purpose of the Study:

    • To investigate the higher-order structure of sea urchin sperm chromatin.
    • To compare its structure to other species with different DNA repeat lengths.

    Main Methods:

    • Electron microscopy (EM) of isolated chromatin.
    • X-ray diffraction (XRD) on partially oriented samples.
    • Low-angle X-ray scattering (LAXS) in vitro and in vivo.

    Main Results:

    • EM revealed 300 Å filaments indistinguishable from chicken erythrocyte chromatin.
    • XRD showed similar nucleosome packing and radial disposition as the chicken erythrocyte solenoid model.
    • LAXS patterns differed, suggesting tighter packing of 300 Å filaments in vivo.

    Conclusions:

    • The solenoid model adequately describes sea urchin sperm chromatin structure despite longer linker DNA.
    • Linker DNA in this chromatin must be bent to accommodate the observed structure.
    • In vivo, sea urchin sperm chromatin filaments are packed more densely than previously thought.

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