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Association Between Proteasome 26S Subunit, Non-ATPase 3 Methylation and Insulin β Cell Apoptosis in Type 2 Diabetic

Guiping Huang1,2, Guodong He1,2, Shaoxian Chen1

  • 1Medical Research Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, People's Republic of China.

Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy
|September 10, 2025
PubMed
Abstract

Insights

Methylation of PSMD3 (proteasome subunit 3) is upregulated in type 2 diabetes mellitus (T2DM) and may influence cell apoptosis and proliferation by affecting USP14 activity. This research explores PSMD3 methylation

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • The role of PSMD3 methylation in type 2 diabetes mellitus (T2DM) pathogenesis is not fully understood.
  • PSMD3 methylation's impact on protein stability and degradation may be significant in T2DM development.

Purpose of the Study:

  • To investigate the molecular and bioinformatic characteristics of PSMD3 methylation in T2DM.
  • To explore the association between PSMD3 methylation and T2DM pathogenesis.

Main Methods:

  • Bioinformatics analyses on T2DM database chip.
  • Establishment of a high glucose-induced T2DM cell model (RIN-m5F cells).
  • Assessment of PSMD3 gene function via overexpression, Western blotting, and flow cytometry.

Main Results:

  • PSMD3 methylation was upregulated in T2DM tissues and correlated with USP14.
  • PSMD3 overexpression decreased apoptosis and increased proliferation in high glucose-treated cells.
  • PSMD3 was linked to USP14 inactivation in high glucose-treated cells.

Conclusions:

  • PSMD3 methylation may influence T2DM development by affecting cell apoptosis and proliferation, potentially through USP14 activation.
  • This study enhances understanding of the molecular mechanisms underlying T2DM pathogenesis related to PSMD3 methylation.
Keywords:
apoptosisinsulin β cellmethylationnon-ATPase 3proteasome 26S subunittype 2 diabetic mellitus

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