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Updated: Jan 18, 2026
Imaging Features of Systemic Sclerosis-Associated Interstitial Lung Disease
Published on: June 16, 2020
Longitudinal Autoantibody and Proteomic Signatures of Disease Progression in Systemic Sclerosis
Muyao Guo1, Sijia Liu1, Jing Huang1
1Department of Rheumatology and Immunology, Provincial Clinical Research Center for Rheumatic and Immunologic Diseases, and National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, China.
Objective:
To evaluate dynamic changes in autoantibody and proteomic profiles in treatment-naïve patients with systemic sclerosis (SSc) and to identify biomarkers and mechanisms associated with disease progression.
Methods:
Serum samples from 30 baseline and 49 follow-up patients with SSc, along with 38 controls, were analyzed. Autoantibody profiles were assessed using an autoantigen microarray targeting 120 autoantibodies, whereas proteomic analysis was conducted via liquid chromatography mass spectrometry in data-independent acquisition mode. Cox proportional hazards models were used to assess associations with disease progression, and Spearman's correlation was applied to analyze antibody-protein interactions.
Results:
Baseline autoantibody profiling identified two patient subgroups: high expression (SSc_high) and low expression (SSc_low), though their clinical characteristics were similar. Longitudinal analysis revealed that 88.9% of patients with rising autoantibody titers experienced disease progression, whereas 60% of those with decreasing titers showed clinical improvement. Notably, increased CENP-B titers (hazard ratio 5.036) were strongly linked to disease progression. Proteomic analysis identified 112 proteins involved in tissue repair and immune modulation in patients with declining titers and clinical improvement, whereas 46 proteins linked to lipid metabolism and oxidative stress were enriched in those with rising titers and disease progression. SERPINA10 emerged as the strongest risk factor for progression, whereas ENTPD5 was most protective. Antibody-protein correlations that highlight key molecular interactions, including SERPINE1 and muscarinic receptor antibodies, were identified.
Conclusion:
Dynamic changes in autoantibody titers, rather than baseline levels, were more strongly associated with SSc trajectory. Proteomic signatures suggest lipid metabolism and oxidative stress as potential drivers, highlighting novel biomarkers and therapeutic targets for personalized SSc management.
