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An Efficient Method for Adenovirus Production
Published on: June 10, 2021
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Replication-competent adenovirus reporters utilizing endogenous viral expression architecture
Claire M O'Brien1, Lorenzo Serra1,2, Molly R Patterson1
1Genome Regulation and Cell Signaling, Ellen and Ronald Caplan Cancer Center, The Wistar Institute, Philadelphia, Pennsylvania, USA.
Journal of Virology
|September 10, 2025
Summary
Researchers developed new adenovirus (Ad) reporter viruses for real-time study of viral gene expression. These advanced tools enable non-destructive tracking of infection dynamics, aiding in the development of novel therapeutics and vaccines.
Area of Science:
- Virology
- Molecular Biology
- Biotechnology
Background:
- Adenoviruses are versatile DNA viruses used in gene therapy and vaccine development.
- Current methods for studying adenoviral gene expression in real time are limited.
Purpose of the Study:
- To create novel fluorescent and bioluminescent reporter viruses for real-time adenoviral gene expression analysis.
- To enable non-destructive, longitudinal tracking of viral infection dynamics in living cells.
Main Methods:
- Utilized the AdenoBuilder reverse genetics system to construct reporter viruses.
- Integrated fluorescent and luciferase reporters into endogenous viral transcriptional units.
- Informed reporter design using high-resolution maps of Ad5 transcription.
Main Results:
- Developed reporter viruses that replicate similarly to wild-type adenoviruses.
- Enabled real-time fluorescent imaging and longitudinal bioluminescent sampling.
- Demonstrated visualization of viral gene expression without exogenous promoters.
Conclusions:
- The new adenovirus reporters offer a modular and tractable platform for studying viral gene regulation and replication.
- These tools facilitate high-throughput screening and broad applications in basic research.
- Facilitates rapid creation of novel infectious and replication-incompetent viral vectors.
