Application of dispersive solid-phase extraction materials based on nucleic acid aptamer-modified magnetic
Liangliang Peng1, Yao Yao1, Meiqi Li1
1Department Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, GDMPA Key Laboratory for Process Control and Quality Evaluation of Chiral Pharmaceuticals, School of Chemistry, South China Normal University, Guangzhou 510006 Guangdong, PR China.
Abstract:
Zearalenone (ZEN), a mycotoxin produced by fungi of the genus Fusarium, is widely present in animal feeds and human foods, posing a serious hazard to human health. Herein, a zearalenone aptamer-functionalized magnetic metal-organic framework material (ZEN-Apt@MMIL-100(Fe)) was constructed and applied for the rapid enrichment of ZEN, coupled with high-performance liquid chromatography (HPLC) for ultrasensitive detection. By a self-templating method, magnetic MIL-100(Fe) was formed by self-assembly of sodium citrate-modified Fe3O4 particles as nuclei with homobenzoic acid, which was bonded to amino-modified zearalenone nucleic acid aptamer via amide reaction to realize a specific recognition function. The magnetic dispersive solid-phase extraction and HPLC method was established using this material, with the linear range of 5 to 200 μg/L (R2 = 0.997) and the limit of detection of 2.92 μg/L. The method was applied to detect ZEN in maize and wheat samples, with spiked recoveries ranging from 81.7% to 98.2% at spiking levels of 25 μg/L, 50 μg/L, and 100 μg/L. The method is able to realize the selective and rapid enrichment and detection of trace ZEN in wheat and maize through the synergistic action of aptamer-specific recognition and magnetic MOFs, which provides a sensitive and reliable analytical strategy for food security monitoring.
