Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

QutRNA2: robust tRNA modification discovery from Nanopore direct tRNA sequencing.

NAR genomics and bioinformatics·2026

Same author

RBM20 isoform regulation by independent transcription start sites adapts alternative splicing in development and disease.

Nature communications·2026

Same author

Author Correction: Pharmacological markers of HIV prevention for oral pre-exposure prophylaxis in men who have sex with men.

Nature communications·2026

Same author

Pharmacological markers of HIV prevention for oral pre-exposure prophylaxis in men who have sex with men.

Nature communications·2026

Same author

CAMK2D causes heart failure in mice with RBM20 cardiomyopathy.

Nature cardiovascular research·2026

Same author

Pharmacokinetics, Pharmacodynamics, Efficacy and Drug Resistance Selection of Injectable Long-Acting Lenacapavir Pre-Exposure Prophylaxis (PrEP) Against HIV.

CPT: pharmacometrics & systems pharmacology·2026

Search research articles

Related Experiment Video

Updated: Jul 6, 2026

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

WarpDemuX-tRNA: barcode multiplexing for nanopore tRNA sequencing.

Wiep van der Toorn^1,2, Isabel S Naarmann-de Vries^3,4, Wang Liu-Wei^1,2,5

¹Systems Medicine of Infectious Disease (P5), Robert Koch Institute, 13353 Berlin, Germany.

Nucleic Acids Research

|September 10, 2025

Summary

WarpDemuX-tRNA is a new tool for multiplexed nanopore sequencing of transfer RNA (tRNA). It enables efficient and accurate identification of tRNA modifications, advancing protein translation research.

More Related Videos

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Related Experiment Videos

Last Updated: Jul 6, 2026

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Area of Science:

Molecular Biology
Genomics
Bioinformatics

Background:

Transfer RNA (tRNA) is crucial for protein translation.
tRNA modifications significantly impact tRNA function.
Nanopore direct RNA sequencing (dRNA-seq) shows potential for detecting tRNA modifications.

Purpose of the Study:

To develop a demultiplexing solution for multiplexed nanopore tRNA sequencing.
To improve the accuracy and efficiency of identifying tRNA modifications using nanopore sequencing.

Main Methods:

Developed WarpDemuX-tRNA, an extension of the WarpDemuX method.
Utilized consensus-based signal analysis with dynamic time warping and barycenter averaging.
Optimized barcode feature generation and identification for tRNA sequencing.

Main Results:

WarpDemuX-tRNA achieved 99% precision and 95% recovery for four barcodes.
Reduced computational complexity and runtime to 6 minutes per million reads.
Outperformed the original WarpDemuX method in tRNA sequencing applications.

Conclusions:

WarpDemuX-tRNA provides a robust solution for high-throughput nanopore tRNA sequencing.
This method facilitates more accessible and cost-effective studies of tRNA modifications.
Enables deeper investigation into the regulatory mechanisms of tRNA modifications.