Generation of humanized bone for disease modeling using porcine adipose tissue-derived extracellular matrix scaffolds...

Human Dental Pulp Stem Cells (hDPSCs) represent a remarkable cell source for tissue engineering and regenerative medicine, offering significant potential for use in personalized medicine and autologous therapies. Decellularized extracellular matrix (ECM)-derived biological scaffolds show excellent properties for supporting cell delivery and growth in both in vitro and in vivo applications. These scaffolds provide essential biochemical cues that regulate cellular functions and offer a more accurate representation of the in vivo environment. Porcine decellularized adipose tissue (pDAT) is a very abundant source of ECM, constituting an ideal material for biologic scaffold preparation. The integration of hDPSCs with pDAT-derived ECM enables the patient-specific generation of diverse humanized tissues and their application in personalized drug screening platforms. This chapter details a three dimensional (3D) culture methodology utilizing hDPSCs and pDAT-derived scaffolds to engineer humanized bone tissue.