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Updated: Jan 18, 2026
Microcrystal Electron Diffraction of Small Molecules
Published on: March 15, 2021
Combination of Microcrystal Electron Diffraction and Solid-State NMR for the Structural Determination of
Hidetomo Yokoo1, Tatsuo Koide1, Tamaki Miyazaki1
1National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki 210-9501, Japan.
Abstract:
The development of analytical techniques applicable to powdered pharmaceutical co-crystals, including those containing excipients, represents a comprehensive strategy for quality control in both drug development and regulatory settings. This study investigates the structural characterization of indomethacin-nicotinamide co-crystals using a combination of microcrystal electron diffraction (microED), solid-state NMR (SSNMR), Raman spectroscopy, and powder X-ray diffraction (PXRD). MicroED analysis revealed the crystal structure of the co-crystal, while SSNMR measurements provided insights into the molecular interactions within the structure. The combination of solid-state NMR and microED with general techniques such as PXRD and Raman spectroscopy is considered useful for confirming crystal properties that cannot be identified by a single method and is applicable to both active pharmaceutical ingredients and final formulations.
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