Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

UDP-glucuronate decarboxylase 1 promotes tumor immune evasion by accelerating KMT2D loss in hepatocellular carcinoma.

Journal for immunotherapy of cancer·2026

Same author

Transient T cell therapies.

Advances in pharmacology (San Diego, Calif.)·2026

Same author

Targeting cholesterol esterification sensitizes liver cancer to CD8<sup>+</sup> T cell attack by impairing metabolic and redox resilience.

Immunity·2026

Same author

Cloning and functional verification of genes LjHD8 and LjMIXTA1 related to trichome development in Lonicera japonica Thunb.

Planta·2026

Same author

OpenIO: An open framework for AI-native immunotherapy.

Cancer cell·2026

Same author

Boundary echo control of S0-mode lamb waves using gradient phononic crystals.

Ultrasonics·2026

Search research articles

Related Experiment Video

Updated: Jan 6, 2026

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

Published on: November 17, 2018

Amplifying antigen-induced cellular responses with proximity labelling.

Shuojun Li¹, Yinghui Men^1,2, Zihan Wang¹

¹Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Key Laboratory of RNA Innovation Science and Engineering, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.

|September 10, 2025

Summary

This summary is machine-generated.

Researchers developed a novel proximity-labeling technology to amplify synthetic antigen clusters on cell surfaces. This method enhances T-cell activation and triggers potent anti-tumor immune responses, leading to tumor eradication and long-term immunity.

More Related Videos

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Published on: April 25, 2018

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

Related Experiment Videos

Last Updated: Jan 6, 2026

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

Published on: November 17, 2018

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Published on: April 25, 2018

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

Area of Science:

Immunology
Biochemistry
Molecular Biology

Background:

Antigen-induced receptor clustering is crucial for cell signaling but often limited by low antigen density.
Naturally occurring antigens, like tumor-associated antigens, may not induce sufficient receptor clustering for robust immune responses.

Purpose of the Study:

To develop a proximity-labeling technology for amplifying synthetic antigen clusters on cell surfaces.
To enhance T-cell receptor (TCR) clustering and downstream signaling for improved anti-tumor immunity.

Main Methods:

Repurposed proximity labeling to create high-density fluorescein probe clusters near target antigens.
Developed in vivo proximity-labeling controlled by red light or ultrasound.
Utilized fluorescein-binding bispecific T-cell engagers to activate T-cells.

Main Results:

Amplified fluorescein clusters effectively engaged TCRs, enhancing T-cell activation and cytotoxicity.
Noninvasive, tissue-selective labeling in mouse tumor models led to rapid tumor eradication.
Induced epitope spreading, promoting systemic immunity and immunological memory.

Conclusions:

Proximity-labeling chemistry offers a versatile strategy to enhance antigen-dependent receptor function.
This approach can be generalized to manipulate cell states and immune responses.
The technology shows promise for cancer immunotherapy by amplifying immune cell activation against tumors.