Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transducer Mechanism: Nuclear Receptors01:31

Transducer Mechanism: Nuclear Receptors

2.4K
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
2.4K
Regulation of Food Intake01:30

Regulation of Food Intake

2.3K
Short-term regulation of food intake primarily involves neural signals from the gastrointestinal (GI) tract, blood nutrient levels, and GI tract hormones. Communication between the gut and brain via vagal nerve fibers plays a significant role in evaluating the contents of the gut. Clinical studies have shown that protein ingestion produces a more prolonged response in these nerve fibers compared to an equivalent amount of glucose. Additionally, the activation of stretch receptors caused by GI...
2.3K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.3K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.3K
Global Regulatory Systems01:28

Global Regulatory Systems

612
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
612
Metabolic States of the Body: Fasting and Starvation01:24

Metabolic States of the Body: Fasting and Starvation

2.7K
During the initial hours of fasting, the body uses up its glycogen stores as an energy source. Once these glycogen reserves are depleted, the body begins breaking down stored triglycerides and structural proteins. During this stage, glycerol becomes a key substrate for gluconeogenesis, while free fatty acids undergo beta-oxidation to provide energy for tissues, such as skeletal muscle. In the fasting state, the body spares protein breakdown as much as possible to conserve muscle and structural...
2.7K
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

592
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
592

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3'-sialyllactose inhibits the migration and invasion of LPS-induced B16F10 mouse melanoma cells through inactivation of the NF-<sub>K</sub>B signaling pathway.

Glycoconjugate journal·2026
Same author

Perillaldehyde reduces mitochondrial oxidative stress damage by regulating KCNT2/NR1D1 protein activity in vascular dementia rats.

Molecular biology reports·2026
Same author

Squalene synthase induces ERα expression via cholesterol supplementation to confer statin resistance in lung cancer cells.

Cancer cell international·2026
Same author

Erratum for Hsu et al., "Evaluating the susceptibility of various common cell lines and assessing inactivation conditions to Mpox virus".

Microbiology spectrum·2026
Same author

Probing Hidden Vortices and Geometrical Effect via Surface-Projected Quasiparticle States.

Nano letters·2025
Same author

Reference range of bone age-based uterine volume in growing girls.

Pediatrics and neonatology·2025
Same journal

Macrophage-secreted brain-derived neurotrophic factor promotes tumor growth in triple-negative breast cancer by inducing axonogenesis.

Cell death and differentiation·2026
Same journal

Species-specific regulation of necroptosis by STK38-dependent RIPK1 phosphorylation.

Cell death and differentiation·2026
Same journal

Ssu72 phosphatase orchestrates obesogenic adipogenesis and metabolic homeostasis during nutrient excess.

Cell death and differentiation·2026
Same journal

SETD7 depletion enhances white adipose browning and ameliorates metabolic disorders in obese mice.

Cell death and differentiation·2026
Same journal

LFPM inhibition of RING1-mediated p53<sup>R175H</sup> degradation drives oncogenesis in p53<sup>R175H</sup>-mutant cancers.

Cell death and differentiation·2026
Same journal

Cell engulfment defines spatially distinct competitive metabolic niches associated with clinical outcomes in colorectal cancer.

Cell death and differentiation·2026
See all related articles

Related Experiment Video

Updated: Jan 18, 2026

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

15.7K

RNF128 regulates the adaptive metabolic response to fasting by modulating PPARα function.

Yu-Lung Lin1,2, Pei-Yao Liu3,4, Yu-Ling Tsai5,6

  • 1The Ph.D. Program for Translational Medicine, College for Medical Science and Technology, Taipei Medical University, Taipei, Taiwan.

Cell Death and Differentiation
|September 10, 2025
PubMed
Summary
This summary is machine-generated.

Researchers discovered that RING finger protein 128 (RNF128) degrades the transcription factor PPARα during fasting. This RNF128-mediated degradation is crucial for metabolic adaptation and lipid regulation in the liver.

More Related Videos

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice
08:06

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice

Published on: November 27, 2019

9.5K
Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

2.8K

Related Experiment Videos

Last Updated: Jan 18, 2026

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

15.7K
Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice
08:06

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice

Published on: November 27, 2019

9.5K
Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

2.8K

Area of Science:

  • Metabolic regulation
  • Molecular biology
  • Lipid metabolism

Background:

  • Peroxisome proliferator-activated receptor alpha (PPARα) regulates key metabolic processes like fatty acid oxidation and ketogenesis during fasting.
  • The precise molecular mechanisms governing PPARα activity, particularly its regulation during nutrient deprivation, are not fully understood.

Purpose of the Study:

  • To identify novel regulators of PPARα function during fasting.
  • To elucidate the role of RING finger protein 128 (RNF128) in PPARα-mediated metabolic adaptation.

Main Methods:

  • * In vitro studies: Investigated the interaction between RNF128 and PPARα, assessing polyubiquitination and degradation of PPARα.
  • * Gene expression analysis: Measured the impact of RNF128 on PPARα target genes, including fibroblast growth factor 21 (FGF21) and lipid metabolism-related genes.
  • * In vivo studies: Utilized RNF128-deficient mice and adeno-associated virus (AAV) serotype 8 mediated overexpression models to assess metabolic parameters during fasting.

Main Results:

  • * Identified RNF128 as a novel PPARα-binding protein that promotes PPARα polyubiquitination and subsequent degradation.
  • * RNF128 overexpression suppressed PPARα activity, inhibiting FGF21 expression and lipid metabolism genes during fasting.
  • * Silencing RNF128 enhanced PPARα-dependent fatty acid oxidation and ketogenesis in starved cells.
  • * In vivo, RNF128 deficiency improved lipid profiles and boosted fatty acid oxidation and ketogenesis during fasting.
  • * AAV8-mediated RNF128 overexpression in mice led to elevated lipid levels and reduced expression of metabolic genes.

Conclusions:

  • RNF128 acts as a critical negative regulator of PPARα during fasting by promoting its degradation.
  • RNF128 plays a vital role in hepatic metabolic adaptation to nutrient deprivation by modulating PPARα activity.
  • RNF128 represents a potential therapeutic target for metabolic disorders associated with impaired fasting response.