Related Experiment Video
Updated: Jan 18, 2026
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Asymmetrically Coordinated CoN5 Sites with Enhanced Oxidase-like Activity for Dual-Mode Colorimetric Sensing of
Huiyun Zhang1, Zigeng Zhao1, Xiaotao Zhang1,2
1State Key Laboratory of Advanced Materials for Intelligent Sensing & Key Laboratory of Organic Integrated Circuit Ministry of Education & Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science & Institute of Molecular Aggregation Science, Tianjin University, Tianjin 300072, China.
Abstract:
The design of efficient and user-friendly methods for nitrite detection is of great significance owing to its critical role in food safety and environmental protection. Herein, we report a novel cobalt single-atom nanozyme (CoN5 SA) featuring a highly asymmetric CoN5 coordination environment. This structural configuration stabilizes high-spin Co2+ species and significantly enhances the oxidase-like activity. Leveraging this nanozyme, a dual-mode colorimetric sensing platform was developed integrating ratiometric detection and smartphone-based visual readout. The ratiometric method exhibited a wide linear range of 0-80 μM with a low detection limit of 0.52 μM, while the smartphone-assisted method showed a linear range of 0-70 μM and a detection limit of 0.87 μM. Both modes exhibited outstanding selectivity, rapid response, and satisfactory recoveries (92.6-97.6%) in real food samples. This work not only elucidates the structure-activity relationship of CoN5 single-atom sites but also presents a robust and user-friendly strategy for on-site nitrite detection.
