Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ancestry-linked IL-10 signaling and macrophage activation modulate fibroblast responses to oxidative stress in a PEG-based microphysiological system.

npj biomedical innovations·2026
Same author

Precision Biomaterials: Incorporating Sex, Age, and Social Descriptors in Biomaterials Research.

ACS biomaterials science & engineering·2026
Same author

Cell line-specific estrogen responses uncover functional sex differences in murine macrophages.

Biology of sex differences·2025
Same author

YIGSR, A Laminin-Derived Peptide, Dictates a Concentration-Dependent Impact on Macrophage Phenotype Response.

Cellular and molecular bioengineering·2024
Same author

Introduction to Biomaterials in Innate Immunity.

Materials advances·2024
Same author

Metformin Treatment of Macrophages Increases Microvessel Growth in Three-Dimensional Hydrogel Coculture.

Tissue engineering. Part A·2024

Related Experiment Video

Updated: Jan 18, 2026

A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties
08:40

A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties

Published on: August 7, 2020

6.7K

Modeling Uterine Fibroids Using Bioengineered Hydrogels.

Allison K Moses1, Miriam Tamaño-Blanco1, Erika Moore1

  • 1Fischell Department of Bioengineering, University of Maryland, College Park, Maryland 20742, United States.

ACS Biomaterials Science & Engineering
|September 11, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a 3D model to study uterine fibroids. This model mimics fibroid tissue, revealing how transforming growth factor beta 3 (TGF-β3) affects fibroblast behavior and extracellular matrix production.

Keywords:
3D in vitro modelextracellular matrixfibroblast activationhydrogeltransforming growth factor betauterine fibroids

More Related Videos

Author Spotlight: In Vitro Hydrogel Model for Glioblastoma Microenvironment Study
10:44

Author Spotlight: In Vitro Hydrogel Model for Glioblastoma Microenvironment Study

Published on: September 22, 2023

2.2K
Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models
10:49

Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models

Published on: June 16, 2022

3.0K

Related Experiment Videos

Last Updated: Jan 18, 2026

A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties
08:40

A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties

Published on: August 7, 2020

6.7K
Author Spotlight: In Vitro Hydrogel Model for Glioblastoma Microenvironment Study
10:44

Author Spotlight: In Vitro Hydrogel Model for Glioblastoma Microenvironment Study

Published on: September 22, 2023

2.2K
Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models
10:49

Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models

Published on: June 16, 2022

3.0K

Area of Science:

  • Biomedical Engineering
  • Gynecology
  • Cell Biology

Background:

  • Uterine fibroids are common gynecological tumors with poorly understood growth mechanisms.
  • Existing in vitro models lack the 3D complexity of fibroid tissue, limiting research.
  • A better model is needed to study fibroid pathogenesis and cellular behavior.

Purpose of the Study:

  • To develop a 3D in vitro model simulating the uterine fibroid microenvironment.
  • To investigate the role of transforming growth factor beta 3 (TGF-β3) in fibroid development.
  • To provide a physiologically relevant platform for studying uterine fibroblast function.

Main Methods:

  • Human uterine fibroblasts were encapsulated in poly(ethylene glycol) (PEG)-based hydrogels.
  • Hydrogels incorporated collagen- and fibronectin-derived peptides to mimic tissue stiffness.
  • Fibroblasts were treated with TGF-β3 to induce fibrotic markers.

Main Results:

  • TGF-β3 treatment increased alpha smooth muscle actin and extracellular matrix protein production.
  • Fibroblasts exhibited increased cell elongation, metabolic activity, and matrix remodeling.
  • The 3D model successfully replicated fibroid-like cellular responses.

Conclusions:

  • The developed 3D hydrogel model effectively mimics the uterine fibroid microenvironment.
  • This model allows for the investigation of cellular mechanisms driving fibroid growth.
  • It offers a promising platform for advancing the understanding of fibroid pathogenesis.