Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Exosomes01:36

Overview of Exosomes

3.5K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Autocatalytic Molecular Sensor Enables Rapid, Sensitive, and One-Pot Detection of Nucleic Acids.

ACS nano·2026
Same author

Allosteric DNAzyme-Enabled Sensitive and Multiplex Detection of Biomarkers for Rapid Diagnosis of Urinary Tract Infections.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Scaffolding Light-Up Aptamers on DNA Nanostructures for Fluorescence Enhancement.

ACS biomaterials science & engineering·2025
Same author

Sorting Extrusion-Prepared Coreless Cell Membrane Vesicles of Mixed Orientations by Functional DNA Probes.

JACS Au·2025
Same author

Quantitative and Multiplexing Analysis of MicroRNAs by Direct Full-Length Sequencing in Nanopores.

Journal of the American Chemical Society·2025
Same author

Visual Detection and Identification of Influenza A Viruses by Nucleic Acid Probe-Enabled Lateral Flow Assay.

Chembiochem : a European journal of chemical biology·2025

Related Experiment Video

Updated: Jan 18, 2026

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles
09:19

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Published on: March 28, 2021

9.6K

DNA nanotechnology-enabled bioanalysis of extracellular vesicles.

Li Pan1, Pengfei Wang1

  • 1Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China. pengfei.wang@sjtu.edu.cn.

Nanoscale Horizons
|September 11, 2025
PubMed
Summary

DNA nanotechnology offers advanced methods for detecting biomarkers in extracellular vesicles (EVs) for liquid biopsy. This review covers DNA-based strategies for analyzing EV proteins and nucleic acids, aiding disease diagnostics.

More Related Videos

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
09:16

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

10.3K
Characterizing Extracellular Vesicles from Biological Fluids
05:07

Characterizing Extracellular Vesicles from Biological Fluids

Published on: February 28, 2025

823

Related Experiment Videos

Last Updated: Jan 18, 2026

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles
09:19

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

Published on: March 28, 2021

9.6K
Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
09:16

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

10.3K
Characterizing Extracellular Vesicles from Biological Fluids
05:07

Characterizing Extracellular Vesicles from Biological Fluids

Published on: February 28, 2025

823

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Diagnostics

Background:

  • Extracellular vesicles (EVs) are crucial biomarkers for liquid biopsy due to their cargo of proteins and nucleic acids.
  • DNA nanotechnology provides programmable, specific, and sensitive tools for analyzing these EV-associated molecules.

Purpose of the Study:

  • To review recent advancements in DNA nanotechnology-based analytical methods for detecting EV-associated proteins and nucleic acids.
  • To discuss the principles, applications, and performance of various DNA-based strategies.

Main Methods:

  • Aptamer-based recognition for target binding.
  • Enzyme-free and enzyme-catalyzed amplification circuits (e.g., HCR, CHA, RCA, CRISPR-Cas).
  • Utilization of DNA nanostructures for signal amplification.

Main Results:

  • Detailed review of diverse DNA nanotechnology strategies for EV biomarker detection.
  • Exploration of integrated multiplexed detection platforms.
  • Summary of key techniques including aptamers, HCR, CHA, RCA, and CRISPR-Cas.

Conclusions:

  • DNA nanotechnology presents a powerful toolkit for sensitive and specific detection of EV biomarkers.
  • Challenges remain in clinical translation, but future perspectives are promising for improved diagnostics.