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Related Experiment Video

Updated: Jul 4, 2026

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

Published on: February 16, 2018

Repopulating Microglia Suppress Peripheral Immune Cell Infiltration to Promote Poststroke Recovery.

Ligen Shi^1,2,3, Lingxiao Lu^1,3, Jun Hu¹

¹Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.

CNS Neuroscience & Therapeutics

|September 11, 2025

Summary

Transiently depleting and repopulating microglia after ischemic stroke reshapes the immune environment, promoting neurofunctional recovery. This approach enhances brain repair and functional outcomes post-stroke.

Keywords:

PLX5622 immunomodulation neuroinflammation replenished microglia

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Last Updated: Jul 4, 2026

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

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Published on: February 16, 2018

Assessing Microglial Phagocytosis of Myelin Debris in vitro Under Repeated Magnetic Stimulation

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Published on: June 17, 2025

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model

Isolation and Flow Cytometric Assessment of Neuroimmune Interactions in a Mini-Stroke Murine Model

Published on: June 20, 2025

Area of Science:

Neuroscience
Immunology
Regenerative Medicine

Background:

Sustained neuroinflammation after ischemic stroke hinders tissue repair and functional recovery.
Novel therapeutic strategies are needed to suppress inflammation and promote neurorestoration.

Purpose of the Study:

To investigate the effects of transient microglia depletion and repopulation on the post-stroke immune microenvironment.
To assess the impact of this immunomodulatory strategy on neurological functional recovery.

Main Methods:

Utilized a microglia depletion-repopulation paradigm using PLX5622 in a post-ischemic stroke model.
Employed single-cell transcriptomics, behavioral testing, cytokine arrays, flow cytometry, and immunofluorescence.

Main Results:

PLX5622 administration promoted neurofunctional recovery by reshaping the immune microenvironment.
Repopulated microglia exhibited a more homeostatic phenotype, reducing pro-inflammatory states.
Observed suppressed T cell exhaustion, limited neutrophil differentiation, and promoted phagocytic macrophages, potentially driven by reduced chemokine production and enhanced blood-brain barrier integrity.

Conclusions:

Transient microglial depletion and repopulation facilitate neurological function recovery post-stroke.
This immunomodulatory strategy shows promise for enhancing functional recovery in ischemic brain injury.
The approach is clinically translatable for improving rehabilitation outcomes.