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Quality Difference Analysis of Pomegranate Peels Based on Multidimensional Fingerprint With Chemical Pattern
Dandan Zheng1,2,3,4, Hui Lv2,3, Xiao Lin5
1College of Life Science, Zaozhuang University, Zaozhuang, China.
Biomedical Chromatography : BMC
|September 11, 2025
Summary
This study developed a method to assess pomegranate peel quality using HPLC-PDA-MS/MS and chemometrics. It successfully classified 47 cultivars into three groups, identifying key chemical markers for quality control.
Area of Science:
- Analytical Chemistry
- Chemometrics
- Pharmacognosy
Background:
- Pomegranate peels are rich in bioactive compounds with medicinal properties.
- Standardized quality assessment methods are crucial for utilizing pomegranate peels effectively, especially in Traditional Chinese Medicine.
- Variability in pomegranate peel composition across cultivars necessitates robust analytical approaches.
Purpose of the Study:
- To develop and validate a comprehensive method for evaluating the quality of pomegranate peels from diverse cultivars.
- To classify pomegranate peel samples based on their chemical fingerprints and identify key chemical markers.
- To establish a predictive model for quality assessment and cultivar identification.
Main Methods:
- High-Performance Liquid Chromatography with Photodiode Array and Mass Spectrometry/Mass Spectrometry (HPLC-PDA-MS/MS) for fingerprinting.
- Chemometric techniques including Hierarchical Cluster Analysis (HCA), Principal Component Analysis (PCA), and Partial Least Square-Discriminant Analysis (PLS-DA) for classification.
- Quantitative analysis of 10 key chemical components.
Main Results:
- HPLC-PDA-MS/MS generated distinct fingerprints with 21 characteristic liquid chromatographic peaks and 26 mass spectra characteristic peaks.
- Chemometric analysis successfully classified 47 pomegranate peel cultivars into three distinct groups.
- Four and three chemical markers were identified and labeled using HPLC and MS/MS models, respectively, enabling cultivar-specific identification.
Conclusions:
- The study presents an effective quantitative profiling method for assessing pomegranate peel quality across multiple active ingredients.
- This approach provides a reliable tool for quality control and prediction of pomegranate peels from various cultivars and production areas.
- The findings offer novel insights for research into Traditional Chinese Medicine and the standardization of herbal medicines.
