Revolutionizing Agriculture With CRISPR Technology: Applications, Challenges, and Future Perspectives.

Area of Science:

• Agricultural Science
• Biotechnology
• Genetics

Background:

• CRISPR technologies offer precise, programmable genetic modifications.
• Applications span crops, livestock, aquaculture, and microbes for sustainable agriculture.

Purpose of the Study:

• To review CRISPR applications in agriculture.
• To highlight advances in sustainable agriculture.
• To examine the evolution and future directions of CRISPR tools.

Main Methods:

• Review of CRISPR applications in various agricultural domains.
• Examination of advanced CRISPR tools like base editing and epigenome modulation.
• Analysis of challenges and emerging directions in CRISPR-enabled agriculture.

Main Results:

• CRISPR accelerates crop trait improvement (drought tolerance, nutrient efficiency, pathogen resistance).
• CRISPR enables disease resistance in livestock and aquaculture, hornless cattle, and faster-growing fish.
• Engineered microbes enhance nitrogen fixation and reduce reliance on agricultural inputs.

Conclusions:

• CRISPR technology significantly enhances agricultural productivity, resilience, and sustainability.
• Challenges include off-target effects, delivery efficiency, and regulatory hurdles.
• AI-integrated platforms and novel Cas variants promise further advancements for global food security by 2050.