Establishment and characterization of muscle cell line of spotted seabass (Lateolabrax maculatus)

Spotted seabass (Lateolabrax maculatus) is an economically important fish species in China. In this study, the muscle of L. maculatus was used as a material, and the muscle cell line was successfully established using the tissue block method. The established muscle cell line exhibited vigorous growth and had been successfully passaged for more than 100 generations, maintaining stable polygonal cell morphology. The cell viability of L. maculatus muscle cell line exhibited a notable increase following a 24-h treatment with 30 $\mu$ M taurine, and the relative expression of myogenic regulatory factors myf5 and myf6 was significantly increased. Therefore, the muscle cell line of L. maculatus was successfully established and served as a valuable resource for conducting fundamental research in L. maculatus, providing essential materials for gene function analysis, cytogenetics, and nutritional metabolism mechanism in L. maculatus.