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Related Experiment Videos

Transferable plasmids mediating multiple-antibiotic resistance in Streptococcus faecalis subsp. liquefaciens.

H P Marder, F H Kayser

    Antimicrobial Agents and Chemotherapy
    |August 1, 1977
    PubMed
    Summary

    Four plasmids were isolated from Streptococcus faecalis. One plasmid, pFK14, confers resistance to multiple antibiotics, including chloramphenicol, erythromycin, streptomycin, and lincomycin, and is transferable to other strains.

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    Area of Science:

    • Molecular Biology
    • Microbiology
    • Genetics

    Background:

    • Streptococcus faecalis subsp. liquefaciens is a bacterium known for its genetic variability.
    • Plasmids are extrachromosomal DNA elements that can confer advantageous traits, such as antibiotic resistance, to their host bacteria.

    Purpose of the Study:

    • To isolate and characterize plasmids from Streptococcus faecalis strain HK187.
    • To identify the specific plasmid responsible for antibiotic resistance.
    • To assess the transferability of these antibiotic resistance traits.

    Main Methods:

    • Isolation of four plasmids from S. faecalis strain HK187.
    • Determination of plasmid molecular weights using neutral sucrose gradients and electron microscopy.
    • Assessment of antibiotic resistance profiles and plasmid transfer frequency via mixed cultures.

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    Main Results:

    • Four plasmids with molecular weights of approximately 36 x 10^6, 31 x 10^6, 26 x 10^6, and 4 x 10^6 were isolated.
    • Plasmid 3 (pFK14), with a molecular weight of 26 x 10^6, was identified as conferring resistance to chloramphenicol, erythromycin, high-level streptomycin, and lincomycin.
    • These four antibiotic resistances were efficiently transferred to a plasmid-free S. faecalis recipient strain (JH2-2).

    Conclusions:

    • Plasmid pFK14 is a key determinant of multiple antibiotic resistances in S. faecalis strain HK187.
    • The genetic elements conferring these resistances are conjugative or mobilizable, facilitating their spread.
    • Understanding plasmid-mediated antibiotic resistance is crucial for combating bacterial infections.