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Adapting a 96-well liquid culture assay to a colonial growth confirmation assay utilizing a 3D printed guide-frame
Micah A Lewis1, Eric S Adams2, Elizabeth A McMillan2
1USDA-Agricultural Research Service, U.S. National Poultry Research Center, Quality and Safety Assessment Research Unit, 950 College Station Road, Athens, GA 30605, United States of America.
Journal of Microbiological Methods
|September 11, 2025
Abstract:
Some bacteria are not suited to assays requiring visual growth in broth; it may be useful to transfer cells from these assays to solid media to confirm growth. Here, we present a novel guide-frame system for accurate transfer of a 96-well assay to solid agar using a replicator.
