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Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Updated: Jan 18, 2026

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Modular, open-sourced multiplexing for democratizing spatial omics.

Nicholas Zhang^1,2,3, Zhou Fang^1,3, Priyam Kadakia^1,3

¹Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA. ahmet.coskun@bme.gatech.edu.

|September 12, 2025

Summary

This summary is machine-generated.

Researchers developed PRISMS, an open-source, automated spatial omics pipeline. This cost-effective solution democratizes multiplex imaging for biological discovery using robotics and streamlined microscopy.

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Last Updated: Jan 18, 2026

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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Area of Science:

Spatial omics
Molecular imaging
Biotechnology

Background:

Spatial omics enables visualization of biomolecules in native tissue context.
High costs of proprietary tools limit accessibility of spatial omics techniques.
Need for cost-effective, reproducible solutions for multiplex imaging.

Purpose of the Study:

Introduce PRISMS (Python-based robotic imaging and staining for modular spatial omics), an open-source automated multiplexing pipeline.
Democratize spatial omics by providing an accessible, reproducible, and cost-effective solution.
Enable high-throughput single-molecule fluorescence imaging and super-resolved imaging.

Main Methods:

Utilized a liquid handling robot with thermal control for automated RNA and protein staining.
Developed modular sample holders and Python control for high-throughput imaging.
Integrated with widefield and confocal microscopes and Nikon NIS Elements software.

Main Results:

Demonstrated PRISMS versatility by imaging tissue slides and adherent cells.
Successfully performed super-resolved imaging, including super-resolution radial fluctuations (SRRF).
Achieved high-throughput, single-molecule fluorescence imaging.

Conclusions:

PRISMS democratizes spatial omics through an accessible, reproducible, and cost-effective platform.
The open-source nature of PRISMS facilitates broader adoption and innovation in spatial biology.
PRISMS empowers researchers to advance spatial biology and make new discoveries.