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Efficient removal of antibody aggregates using TOYOPEARL MX-Trp-650M mixed-mode resin under salt or pH gradient
Jinyi Zhang1, Penglong Zhang1, Yifeng Li1
1Downstream Process Development, WuXi Biologics, Waigaoqiao Free Trade Zone, Shanghai 200131, China.
Background:
TOYOPEARL MX-Trp-650M is a mixed-mode resin (Tosoh Bioscience, Japan), which mediates both cation exchange and hydrophobic interactions. While mixed-mode resins are generally effective at removing antibody aggregates, reports specifically evaluating the application of MX-Trp-650M for this purpose remain limited. A previous study suggested that effective separation of monomeric and aggregated antibodies using MX-Trp-650M was achieved only under dual-gradient elution with pH and salt.
Objective:
This study aimed to further evaluate MX-Trp-650M's aggregate separation potential under various elution conditions.
Methods:
In an antibody purification case where aggregates were the predominant byproducts, both Capto MMC ImpRes and MX-Trp-650M were evaluated as the first polishing step following Protein A capture. Aggregate separation was monitored and assessed using native gel electrophoresis and size-exclusion chromatography-high-performance liquid chromatography.
Results:
MX-Trp-650M marginally outperformed Capto MMC ImpRes and achieved excellent aggregate clearance under either salt or pH mono-gradient elution.
Conclusion:
Combining the results from prior studies, the current data confirm the strong aggregate separation capability of MX-Trp-650M. The results also suggest that the optimal elution conditions for efficacious separation may vary across different antibody purification scenarios.
