Ex vivo human brain volumetry: Validation of MRI measurements
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View abstract on PubMed
Summary
This summary is machine-generated.This study validated MRI brain volumes using ex vivo specimens and water displacement. Automatic T1-weighted MRI segmentation showed accuracy comparable to the gold standard, suggesting clinical MRI brain volumes are reliable.
Area Of Science
- Neuroimaging
- Medical Physics
- Anatomy
Background
- Accurate human brain volume measurement is crucial for clinical research.
- Current in vivo MRI volumetry tools lack validation against a gold standard.
- Ex vivo validation offers a unique opportunity to assess MRI accuracy.
Purpose Of The Study
- To validate in vivo MRI brain volume measurements using ex vivo, in situ specimens.
- To compare MRI-derived volumes with the gold-standard water displacement method (WDM).
- To assess the accuracy of different MRI sequences and segmentation techniques.
Main Methods
- Acquired 3T MRI scans (T2-weighted, T1-weighted, MP2RAGE) of seven fixed anatomical heads.
- Segmented gray and white matter using manual thresholding and deep learning (SynthSeg).
- Measured extracted brain volumes using WDM after fixation and dissection.
Main Results
- All tested MRI volumetry methods showed significant differences compared to WDM (p < 0.001).
- Automatic T1-weighted MRI segmentation (SynthSeg) was the only method not significantly different from WDM.
- Mean volumes ranged from 1020.29 cm³ (manual T2) to 1156.18 cm³ (automatic MP2RAGE-2nd inversion).
Conclusions
- SynthSeg provides accurate brain volume measurements on ex vivo, in situ T1-weighted MRI.
- Clinical MRI brain volumes are likely sufficiently accurate for most studies.
- Potential underestimation of in vivo brain volumes may occur depending on the MRI sequence used.
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