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Pseudospirochaetes in peritoneal exudates of mice injected with Bacillus aneurinolyticus
Journal of General Microbiology
|November 1, 1985
Abstract:
Bodies initially thought to be spirochaetes were observed in the ascites of mice injected intraperitoneally with Bacillus aneurinolyticus (KA bacillus). Electron microscopy revealed that these bodies were in fact 'pseudospirochaetes' originating from the flagellar bundles of the bacilli.
Insights
Researchers identified pseudospirochaetes, not spirochaetes, in mice injected with Bacillus aneurinolyticus. These structures originated from the flagellar bundles of the bacteria, revealed by electron microscopy.
Area of Science:
- Microbiology
- Bacteriology
- Microscopy
Background:
- Initial observations suggested the presence of spirochaetes in mouse ascites fluid.
- Bacillus aneurinolyticus, also known as the KA bacillus, was used for intraperitoneal injections in mice.
Purpose of the Study:
- To clarify the nature of the bodies observed in the ascites of mice injected with Bacillus aneurinolyticus.
- To determine if the observed bodies were indeed spirochaetes or a different microbial structure.
Main Methods:
- Intraperitoneal injection of mice with Bacillus aneurinolyticus.
- Analysis of ascites fluid using electron microscopy.
Main Results:
- Bodies initially mistaken for spirochaetes were identified.
- Electron microscopy confirmed these bodies as 'pseudospirochaetes'.
- Pseudospirochaetes were found to originate from the flagellar bundles of Bacillus aneurinolyticus.
Conclusions:
- The observed structures in the ascites of mice injected with Bacillus aneurinolyticus were not spirochaetes.
- These structures, termed 'pseudospirochaetes', are derived from the flagellar apparatus of Bacillus aneurinolyticus.