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Related Experiment Video

Updated: Jan 18, 2026

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Immunoglobulin-Directed Phase Separation for Cell-Specific Modulation of Receptor Signaling.

Weishuai Ren1, Peiyuan Zheng1, Ziting Zhou1

  • 1School of Pharmaceutical Sciences, MOE Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology, Tsinghua University, Beijing 100084, China.

Journal of the American Chemical Society
|September 12, 2025
PubMed
Summary
This summary is machine-generated.

Researchers engineered a novel phase separation system using antibody-peptide interactions. This system specifically targets cancer cells, inducing apoptosis and inhibiting tumor growth for potential therapeutic applications.

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Area of Science:

  • Cellular Biology
  • Biochemistry
  • Immunology

Background:

  • Liquid-liquid phase separation is crucial for cellular functions and signaling pathway modulation.
  • Membrane-associated signaling complexes rely on phase separation for spatiotemporal control.

Purpose of the Study:

  • To engineer a novel phase separation system for targeted cellular signaling modulation.
  • To investigate the therapeutic potential of an immunoglobulin-directed phase separation system for cancer treatment.

Main Methods:

  • Engineered a phase separation system using a mutant Fc fragment (human IgG1) and a specific peptide.
  • Tethered phase separation scaffolds to nanobodies targeting CXCR4 and DR5 cell-surface receptors.
  • Evaluated the system's efficacy in activating DR5 signaling and inducing tumor cell apoptosis in a xenograft model.

Main Results:

  • Successfully engineered a phase separation system based on antibody-peptide multivalent interactions.
  • Achieved specific activation of DR5 signaling in CXCR4+ cells.
  • Demonstrated efficient tumor cell apoptosis and inhibition of tumor growth in vivo.

Conclusions:

  • Immunoglobulin-directed phase separation systems offer a novel strategy for cell-specific signaling modulation.
  • This engineered system shows potential for scientific research and therapeutic applications in cancer therapy.
  • Phase separation systems can be utilized for targeted apoptosis induction and tumor growth inhibition.