Ratiometric electrochemical sensor based on metal-organic framework and nanosilver as response signals for cTnI...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Nanotechnology

Background:

• Cardiac troponin I (cTnI) is a critical biomarker for myocardial injury.
• Existing detection methods may lack the required sensitivity or speed for early diagnosis.

Purpose of the Study:

• To develop a ratiometric electrochemical aptasensor for ultrasensitive cTnI detection.
• To utilize Zirconium-metal organic framework (UiO-66) and silver nanoparticles (AgNPs) for enhanced signal amplification.

Main Methods:

• Fabrication of a ratiometric electrochemical aptasensor.
• Utilizing UiO-66 and AgNPs as electrochemical signaling tags.
• Employing aptamer binding, TdT enzyme, and SI-RAFT polymerization for signal amplification.

Main Results:

• Achieved an ultrasensitive detection range for cTnI from 1 × 10⁻³ to 1 × 10² ng mL⁻¹.
• Obtained a low detection limit of 20.53 fg mL⁻¹.
• Demonstrated excellent performance in detecting cTnI in human serum samples.

Conclusions:

• The developed aptasensor offers a highly sensitive and reliable method for cTnI detection.
• The sensor exhibits significant potential for clinical diagnosis of myocardial injury.
• The combination of UiO-66 and AgNPs provides effective signal amplification for biosensing applications.