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Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
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Inter-droplet gas diffusion in segmented-flow analysis for ammonium determination.

Xiaodi Yan1, Yiyu Jiang1, Jin Zhang1

  • 1School of Life and Environmental Sciences, Guangxi Key Laboratory of Automatic Detecting Technology and Instruments, Guilin University of Electronic Technology, Guilin, 541004, Guangxi, China.

Talanta
|September 12, 2025
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Summary

This study introduces a novel oil-based droplet system for precise ammonium determination. The innovative approach enhances sample analysis throughput and accuracy for volatile compounds.

Keywords:
Ammonium determinationDroplet-based flow analyzerFluorinated oilGas diffusionLiquid membrane

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Area of Science:

  • Analytical Chemistry
  • Environmental Science

Background:

  • Ammonium determination commonly relies on gas diffusion across membranes or air gaps.
  • Traditional methods face challenges with sample throughput and interference in complex matrices.

Purpose of the Study:

  • To develop a novel donor-oil-acceptor droplet system for enhanced ammonium determination.
  • To create an automated flow-through analyzer for improved sample analysis.
  • To evaluate the system's performance, including detection limits and interference mitigation.

Main Methods:

  • Utilized immiscible fluorinated oils (e.g., FC-40) as liquid membranes to segment donor and acceptor droplets.
  • Developed an automated flow-through analyzer with peristaltic micro-pumps for generating paired droplets.
  • Employed bromothymol blue (BTB) for absorbance detection and o-phthalaldehyde (OPA)-sulfite reagent for fluorescence detection.

Main Results:

  • Achieved limits of detection of 14 μmol L⁻¹ (absorbance) and 2 μmol L⁻¹ (fluorescence).
  • Demonstrated minimal interference from alkaline earth metals.
  • Showcased effective mitigation of amine interference using the OPA-sulfite reagent.

Conclusions:

  • The proposed droplet-based system offers an innovative and versatile platform for volatile compound analysis.
  • The system enhances sample throughput and accuracy in complex matrices.
  • This method provides a sensitive and selective approach for ammonium determination.