Real-Time Instrument Control across Multiple Orbitrap Platforms through a Single Software Interface

Area of Science:

• Analytical Chemistry
• Instrument Engineering
• Software Development

Background:

• Real-time spectral analysis and instrument control enhance mass spectrometer capabilities.
• Thermo Fisher Scientific's Instrument Application Programming Interface (IAPI) allows customized application development.
• Current IAPI versions are instrument-specific, requiring separate development for different mass spectrometer platforms (e.g., Exploris, Tribrid).

Purpose of the Study:

• To present Helios, a unified API that simplifies the development of real-time mass spectrometry applications.
• To enable a single codebase for applications compatible with both Exploris and Tribrid instrument platforms.
• To increase the number of supported instruments for real-time control applications.

Main Methods:

• Developed Helios, a unified API that abstracts the platform-specific IAPI for Exploris and Tribrid instruments.
• Implemented automatic resolution of metadata differences from real-time data streams.
• Demonstrated sending customized instrument control commands compatible with both platforms.

Main Results:

• Helios provides a single interface with IAPI-like syntax for Exploris and Tribrid instruments.
• Two example applications were created using Helios, demonstrating cross-platform functionality from a single codebase.
• The developed applications operated identically on both Exploris and Tribrid instruments.

Conclusions:

• Helios simplifies the development of real-time instrument control applications for mass spectrometry.
• Helios increases the compatibility of applications across different mass spectrometer instrument platforms.
• This unified API approach eliminates the need for platform-specific code, streamlining research and development.