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Related Experiment Video

Updated: Jan 18, 2026

Low-Cost Gait Analysis for Behavioral Phenotyping of Mouse Models of Neuromuscular Disease
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Systematic gait analysis in alpha-synuclein transgenic line 62 mice using the CatWalk.

Karima Schwab1, Lianne Robinson1, Jack Bray1

  • 1School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK.

Behavioural Brain Research
|September 12, 2025
PubMed
Summary

Parkinson's disease (PD) mouse models show impaired gait, with deficits worsening with age. These findings highlight α-synuclein's role in motor control and suggest gait analysis as a key tool for PD research.

Keywords:
Alpha-synucleinCatWalkCoordinationGaitLocomotionParkinson’s disease

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Area of Science:

  • Neuroscience
  • Animal Models
  • Biomedical Engineering

Background:

  • Alpha-synuclein aggregation is a hallmark of Parkinson's disease (PD), causing motor and gait impairments.
  • Rodent gait assessment models are crucial for studying pre-clinical PD.
  • Line 62 (L62) transgenic mice expressing human alpha-synuclein serve as a pre-clinical model for PD and synucleinopathies.

Purpose of the Study:

  • To systematically assess gait performance in L62 transgenic mice using the CatWalk automated gait analysis system.
  • To investigate gait abnormalities in a pre-clinical mouse model of Parkinson's disease.
  • To evaluate the impact of age and sex on gait deficits in L62 mice.

Main Methods:

  • Utilized the CatWalk automated gait analysis system to measure gait parameters in L62 transgenic mice and wild-type (WT) littermates.
  • Compared gait performance between male and female mice of different ages (6 and 12 months).
  • Analyzed spatial and temporal interlimb coordination, stride length, base of support, and running speed.

Main Results:

  • L62 mice exhibited impaired gait, including reduced running time, smaller base of support, and interlimb coordination deficiencies compared to WT mice.
  • Gait deficits were more pronounced in older (12-month-old) L62 mice, particularly males.
  • Kinetic and coordination deficits were observed in both sexes by 6 months, with spatial deficits emerging earlier in males and temporal deficits in females.

Conclusions:

  • Gait performance is significantly impaired in L62 alpha-synuclein transgenic mice, serving as a valid pre-clinical model for Parkinson's disease.
  • Age exacerbates gait impairments, with deficits being most severe in aged male L62 mice.
  • Sex-specific differences in the emergence of spatial and temporal gait deficits were observed, suggesting complex disease progression pathways.