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Related Experiment Video

Updated: Jan 18, 2026

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
13:15

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

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Lab-on-PCB with integrated DNA amplification and electroanalytical detection for point-of-care diagnostics.

Seshagopalan Thorapalli Muralidharan1, Martin Hanze2, Alar Ainla3

  • 1Department of Engineering Design, KTH Royal Institute of Technology, Brinellvägen 83, 10044, Stockholm, Sweden.

Scientific Reports
|September 12, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a low-cost, portable lab-on-PCB device for rapid nucleic acid amplification tests (NAATs). The novel system enables sensitive detection of SARS-CoV-2 at the point-of-care, overcoming limitations of traditional diagnostic methods.

Keywords:
Electrochemical biosensor.Lab-on-PCB.Nucleic acid amplification test (NAAT).Point-of-care.Printed circuit board (PCB)

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Area of Science:

  • Biotechnology
  • Medical Diagnostics
  • Electrochemistry

Background:

  • Nucleic acid amplification tests (NAATs) are crucial for diagnostics but often require complex laboratory equipment.
  • Quantitative PCR (qPCR) is limited to centralized labs due to its complexity and cost.
  • Isothermal amplification methods like Loop-mediated Isothermal Amplification (LAMP) offer potential for point-of-care (POC) applications.

Purpose of the Study:

  • To develop a novel, low-cost, and portable lab-on-PCB device for nucleic acid amplification and electrochemical detection.
  • To adapt reverse transcription LAMP (RT-LAMP) for SARS-CoV-2 detection using a printed circuit board (PCB) platform.
  • To enable rapid and sensitive NAATs at the point-of-care.

Main Methods:

  • A novel lab-on-PCB system utilizing two disposable PCB chips for heating, amplification (RT-LAMP), and electrochemical detection (Cyclic Voltammetry).
  • Integration of a redox-active intercalator for sensitive electrochemical readout.
  • Connection to a compact, low-cost electronic device for system control and data acquisition.

Main Results:

  • Successful rapid (under 1.5 hours) nucleic acid amplification and detection of SARS-CoV-2.
  • Achieved high sensitivity with a target concentration as low as 10 copies per reaction.
  • Demonstrated a cost-effective solution with disposable PCB chips and a compact electronic reader.

Conclusions:

  • The developed lab-on-PCB device offers a promising integrated solution for portable and accessible NAATs.
  • This technology represents a significant advancement towards decentralized diagnostics, particularly for infectious diseases.
  • The system's low cost and ease of use facilitate widespread adoption in point-of-care settings.