Comparative Analysis of Gastrointestinal Morphology and Enteric Nervous System Organization in Mallard, Tufted Duck,...

Ducks exhibit substantial ecological and dietary diversity, which drives morphological and functional adaptations in their digestive systems. This study analyzed the small intestine and cecum of three wild duck species: Mallard (Anas platyrhynchos), Tufted Duck (Aythya fuligula), and Green-Winged Teal (Anas crecca) collected post-mortem. Histomorphometric analysis and immunohistochemistry (IHC) with the pan-neuronal marker HuC/D were performed. The Tufted Duck showed the thickest intestinal muscle layers, particularly in the duodenum and ileum, and the largest enteric ganglia, indicating adaptation to a fibrous and protein-rich diet. The Mallard displayed the longest villi and deepest crypts, consistent with its omnivorous diet rich in plant material. The Green-Winged Teal, which consumes highly digestible insect-rich food, had the shortest villi and thinnest muscle layers. Differences in enteric ganglion size and organization among species suggest varying neuroregulatory demands in different gut segments. These findings confirm that intestinal morphology and enteric nervous system (ENS) structure are tightly linked to dietary specialization and ecological strategies. The results highlight the high adaptive plasticity of the avian digestive system in response to feeding behavior.