Reducing Hormonal Inputs in Rabbit Reproduction: Physical Ovulation Induction with a 3D-Printed Cannula.

Area of Science:

• Reproductive Biology
• Animal Science
• Biotechnology

Background:

• Hormonal induction of ovulation in rabbits often relies on gonadotropin-releasing hormone (GnRH) analogues.
• Reducing hormone use in livestock production is a key goal for sustainable and ethical practices.
• Novel methods for ovulation induction are needed to improve efficiency and animal welfare.

Purpose of the Study:

• To evaluate the efficacy of a 3D-printed cannula for inducing ovulation in female rabbits.
• To compare a physical stimulation technique using the cannula with hormonal induction.
• To assess fertility outcomes in different physiological states of female rabbits.

Main Methods:

• 325 inseminations were conducted in nulliparous, multiparous lactating, and multiparous non-lactating does.
• Two insemination methods were compared: 3D-printed cannula physical stimulation versus hormonal induction (buserelin acetate).
• Females were either synchronized with equine chorionic gonadotropin (eCG) or left untreated; delivery rates and litter sizes were recorded.

Main Results:

• The control group (hormonal induction) achieved a higher delivery rate (79%) compared to the 3D-printed cannula group (65%).
• The insemination procedure significantly impacted delivery rates.
• Physical stimulation via the cannula demonstrated the potential to induce ovulation without exogenous hormones.

Conclusions:

• Physical stimulation using a 3D-printed cannula can induce ovulation in rabbits, offering a hormone-free alternative.
• This approach presents a promising, welfare-friendly option for rabbit artificial insemination.
• Further research into cannula design and technique optimization could enhance results, particularly for less responsive animals.