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Updated: Jan 18, 2026

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis
Published on: May 10, 2020
Enhancing Biological Nitrogen Fixation Through Diverse Pasture Swards
Rukshagini Sutharsan1, Paramsothy Jeyakumar1, Lucy Burkitt1
1School of Agriculture and Environment, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand.
Diverse pastures enhance biological nitrogen fixation (BNF) and yield compared to standard pastures. Strategic management can boost seasonal BNF, supporting sustainable agriculture and reducing synthetic nitrogen needs.
Area of Science:
- Agricultural Science
- Agronomy
- Soil Science
Background:
- Regenerative agriculture utilizes diverse pasture species for sustainable production.
- Incorporating legumes into pastures can increase biological N fixation (BNF), reducing synthetic N fertilizer reliance.
Purpose of the Study:
- Quantify BNF in diverse vs. standard pastures.
- Assess legume performance for improved N supply and pasture quality.
- Evaluate year-round seasonal BNF through various indicators.
Main Methods:
- Field study comparing diverse (9 species) and standard (ryegrass/clover) pastures.
- Assessed soil N status, nodulation, plant composition, and 15N natural abundance.
- Evaluated dry matter production and nitrogen yield.
Main Results:
- Diverse pastures yielded 5.4% more dry matter and 9.3% higher N yield than standard pastures.
- Soil mineral N levels were similar between pasture types.
- BNF increased 3-fold in diverse pastures from winter to summer, compared to 1.5-fold in standard pastures.
- Optimal BNF occurred at moderate clover proportions (up to 30%) in both systems.
Conclusions:
- Diverse pastures can enhance seasonal BNF and overall productivity.
- Strategic management is key to maximizing BNF benefits in diverse swards.
- These findings support the use of diverse pastures for sustainable N management in agriculture.
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