Oyster Fermentation Broth Alleviated Tripterygium-Glycosides-Induced Reproductive Damage in Male Rats

Area of Science:

• Reproductive Biology
• Nutritional Science
• Toxicology

Background:

• Tripterygium glycosides (TG) are known to induce oxidative stress and reproductive damage in males.
• Oyster fermentation broth (OFB) is a novel preparation from oysters with potential bioactive properties.
• Investigating OFB's protective effects against TG-induced toxicity is crucial for understanding its health benefits.

Purpose of the Study:

• To evaluate the efficacy of OFB in mitigating TG-induced reproductive damage in male rats.
• To explore the underlying mechanisms, particularly the antioxidant and spermatogenic effects of OFB.

Main Methods:

• OFB was prepared and analyzed for its bioactive components and in vitro antioxidant capacity.
• Male rats were induced with TG to model reproductive damage.
• Rats were administered varying doses of OFB, and reproductive parameters, serum testosterone, and testicular histology were assessed.
• Enzyme activities (CAT, SOD, GSH-PX) and lipid peroxidation (MDA) in testes were measured.

Main Results:

• OFB exhibited significant in vitro antioxidant activity.
• OFB administration dose-dependently improved testis and seminal vesicle indices, sperm count, and testosterone levels in TG-treated rats.
• OFB reduced sperm malformation rates and ameliorated testicular histological damage by increasing spermatogenesis.
• OFB enhanced antioxidant enzyme activities and reduced lipid peroxidation in rat testes.

Conclusions:

• OFB effectively alleviates TG-induced reproductive toxicity in male rats, primarily through its potent antioxidant properties.
• OFB demonstrates potential as a functional food ingredient for enhancing male reproductive health.
• The findings support the development of oyster-derived products for managing reproductive dysfunction.