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Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: May 1, 2026

$Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2$

Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2

Published on: December 8, 2016

3D Measurement of Neutron-Induced Tracks Using Confocal Microscopy.

Gavin K Gillmore^1,2, David Wertheim², Alan Flowers²

¹School of Science, Bath Spa University, Bath BA2 9BN, UK.

Sensors (Basel, Switzerland)

|September 13, 2025

Summary

This summary is machine-generated.

We pioneered a 3D confocal microscopy technique to image neutron tracks in Solid-State Nuclear Track Detectors (SSNTDs). This method successfully visualizes and measures the dimensions of these tracks, aiding in neutron detection analysis.

Keywords:

Solid-State Nuclear Track Etch Detectors laser confocal microscopy neutrons

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Area of Science:

Nuclear Physics and Instrumentation
Materials Science and Engineering

Background:

Solid-State Nuclear Track Detectors (SSNTDs) are crucial for radiation detection.
Characterizing neutron-induced tracks is essential for dosimetry and nuclear physics applications.
Existing imaging techniques may not fully capture the 3D geometry of tracks.

Purpose of the Study:

To present a novel 3D confocal microscopy technique for imaging neutron-induced tracks in SSNTDs.
To demonstrate the capability of this technique in measuring track dimensions and depth.
To assess the impact of neutron source orientation on track characteristics.

Main Methods:

Utilized a pioneered 3D confocal microscopy technique for alpha-track imaging.
Employed an Olympus LEXT laser scanning confocal microscope to image CR39-based SSNTDs.
Exposed detectors to an 241Am(Be) neutron source at varying angles (0°, 30°, 60°).

Main Results:

Successfully imaged the 3D surface of neutron-induced recoil proton tracks.
Measured track depths with a median of 3.07 μm (range: 0.98–8.34 μm).
Determined track width (median 7.49 μm) and breadth (median 8.41 μm) in plan view.

Conclusions:

The developed confocal microscopy approach effectively images 3D tracks in SSNTDs.
This method enables precise measurement of track dimensions and depth.
The technique facilitates the identification of angled tracks, enhancing neutron detection capabilities.