Modeling and Validation of Oocyte Mechanical Behavior Using AFM Measurement and Multiphysics Simulation.

Area of Science:

• Biophysics
• Cell Mechanics
• Finite Element Analysis

Background:

• Oocyte mechanical properties are crucial for quality assessment.
• Simulating oocyte deformation requires distinct models for cell-tool and cell-fluid interactions.
• Understanding intracellular mechanical responses provides insights into oocyte viability.

Purpose of the Study:

• To develop and validate mechanical models for simulating porcine oocyte deformation under various conditions.
• To investigate the mechanical properties of the zona pellucida (ZP) and cytoplasm.
• To establish a foundation for improved oocyte quality assessment.

Main Methods:

• Developed a layered finite element (FE) model incorporating viscoelastic properties of ZP and cytoplasm.
• Utilized Atomic Force Microscopy (AFM) to measure Young's modulus and creep behavior.
• Employed a three-phase flow model to simulate oocyte-fluid interactions in microfluidic channels.

Main Results:

• Determined Young's modulus for ZP (7 kPa) and cytoplasm (1.55 kPa).
• Achieved high accuracy (5.2% error) in simulating oocyte deformation through micropipettes using the FE model.
• Validated the three-phase flow model's efficacy in simulating oocyte deformation in microfluidic channels.

Conclusions:

• The developed mechanical models accurately predict oocyte deformation during micromanipulation and fluid flow.
• Mechanical characterization of oocyte subcomponents is essential for accurate modeling.
• This research offers a valuable tool for oocyte quality assessment and cell mechanics studies.