Related Experiment Video

Updated: Jan 18, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method

Published on: October 7, 2025

348

Dysregulation of MicroRNAs in Hepatocellular Carcinoma: Targeting Oncogenic Signaling Pathways for Innovative

Yusra Zarlashat1, Judit Halász2, Edit Dósa3

  • 1Department of Biochemistry, Government College University Faisalabad, Faisalabad 38000, Pakistan.

International Journal of Molecular Sciences
|September 13, 2025
PubMed

Insights

MicroRNAs (miRNAs) regulate hepatocellular carcinoma (HCC) development by controlling key signaling pathways. miRNA-based therapies show promise for diagnosing and treating HCC, offering new personalized treatment strategies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Hepatocellular carcinoma (HCC) is a major global cancer with high mortality.
  • Oncogene activation and tumor suppressor gene loss drive HCC.
  • MicroRNAs (miRNAs) are crucial regulators of HCC cell proliferation, apoptosis, and metastasis.

Purpose of the Study:

  • To review miRNA's role in regulating HCC signaling pathways.
  • To highlight the diagnostic and therapeutic potential of miRNAs in HCC.
  • To summarize recent advances in miRNA-based HCC treatment strategies.

Main Methods:

  • Literature review of preclinical and clinical studies on miRNAs in HCC.
  • Analysis of miRNA involvement in key signaling pathways (PI3K/Akt/mTOR, Hippo-YAP/TAZ, Wnt/β-catenin, RAS/MAPK, p53).
  • Evaluation of miRNA-based diagnostic biomarkers and therapeutic approaches.

Main Results:

  • miRNAs act as tumor suppressors or oncomiRs, influencing HCC progression.
  • Dysregulated miRNAs in tissues and body fluids are potential biomarkers.
  • miRNA-based strategies (restoring tumor suppressors, inhibiting oncomiRs) show efficacy in preclinical HCC models.
  • Combination therapies and targeted delivery enhance miRNA therapeutic effects.

Conclusions:

  • miRNAs are critical regulators of HCC biology and signaling pathways.
  • miRNAs represent promising biomarkers for HCC early detection and prognostication.
  • miRNA-based therapeutics offer potential for personalized HCC treatment and overcoming resistance.
Keywords:
biomarkershepatocellular carcinomamicroRNAoncomiRsignaling pathwaystherapeutic targetstumor suppressor microRNA

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