Sulfate Deficiency-Responsive MicroRNAs in Tomato Uncover an Expanded and Functionally Integrated Regulatory Network
Contact us if these videos are not relevant.
Contact us if these videos are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.Plant microRNAs (miRNAs) regulate responses to sulfate deficiency. Tomato miRNAs show organ-specific regulation, controlling targets involved in nutrient transport and metabolism.
Area Of Science
- Plant Molecular Biology
- Plant Physiology
- Genomics
Background
- Sulfate availability is crucial for plant growth.
- The role of microRNAs (miRNAs) in plant sulfate deficiency responses is not well understood.
Purpose Of The Study
- To investigate the temporal and organ-specific regulation of miRNAs in tomato (Solanum lycopersicum) roots and leaves under sulfate deficiency.
- To identify novel sulfate deficiency-responsive miRNAs and their targets.
Main Methods
- Temporal analysis of miRNA expression in tomato roots and leaves.
- Updated miRNA annotation using the SL4.0 genome.
- Integration with transcriptomic data and Degradome-seq analysis.
Main Results
- Identified 40 differentially expressed miRNAs, including 2 novel tomato-specific miRNAs.
- Demonstrated time- and organ-specific miRNA regulation.
- miR395 targets genes involved in sulfate transport, assimilation, redox homeostasis, photosynthesis, and chloride transport.
- Observed organ-specific target regulation: repression in leaves and upregulation in roots.
Conclusions
- miRNAs play a critical role in fine-tuning organ-specific metabolic reprogramming during nutrient stress.
- Tomato exhibits a broader functional repertoire of miRNAs in response to sulfate deficiency compared to Arabidopsis thaliana.
- These findings expand the understanding of regulatory mechanisms underlying plant sulfate deficiency.
Contact us if these videos are not relevant.
Contact us if these videos are not relevant.
Related Concept Videos
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to...
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...