Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

987
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
987
The Central Dogma01:20

The Central Dogma

32.1K
The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
32.1K
Recombinant DNA01:09

Recombinant DNA

101.7K
Overview
101.7K
Vaccinations01:51

Vaccinations

51.3K
Overview
51.3K
Cancer Vaccines01:30

Cancer Vaccines

974
Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...
974
Synthetic Biology02:55

Synthetic Biology

5.5K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In Vivo mRNA-Lipid Nanoparticle CAR-T Cell Engineering: Advances, Challenges, and Clinical Translation.

Biomedicines·2026
Same author

MOG-Peptide Synergizes With Rapamycin to Drive CD4<sup>+</sup> T Cells Into Protective Antigen-Specific Tregs in EAE.

Immunology·2026
Same author

Nanoplasmid PSMA DNA vaccine primes antitumor immunity and enhances response to PD-1 blockade against prostate cancer.

Cancer letters·2026
Same author

LGALS9 blockade augments vaccine-induced immune responses against prostate cancer.

Journal for immunotherapy of cancer·2026
Same author

VISTA neutralization by immunization reprograms immunosuppression and augments vaccine efficacy in renal carcinoma.

Journal for immunotherapy of cancer·2026
Same author

Biodegradable Aurum: Gold Nanosheets Undergo Biodegradation by Neutrophil Myeloperoxidase.

Small science·2026
Same journal

RETRACTED: Kim et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Regulating the Visceral Adipose-Tissue Function. <i>Int. J. Mol. Sci.</i> 2017, <i>18</i>, 846.

International journal of molecular sciences·2026
Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026
Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026
Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026
Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026
Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
See all related articles

Related Experiment Video

Updated: Jan 18, 2026

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes
08:27

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

5.0K

DNA Vaccines in the Post-mRNA Era: Engineering, Applications, and Emerging Innovations.

Praveen Neeli1, Dafei Chai2, Debanjana Roy3

  • 1Department of Molecular Oncology and Cancer Biology and Evolution Program, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA.

International Journal of Molecular Sciences
|September 13, 2025
PubMed
Summary
This summary is machine-generated.

DNA vaccines, enhanced by synthetic biology, offer improved potency and stability for infectious diseases and cancer immunotherapy. Innovations like self-amplifying DNA and DNA-encoded monoclonal antibodies position them as a key future platform.

Keywords:
DNA vaccinescancerimmunogenicitylipid-nanoparticlesmRNA vaccinesmonoclonal antibodies

More Related Videos

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
10:16

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Published on: August 17, 2022

3.8K
A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

3.0K

Related Experiment Videos

Last Updated: Jan 18, 2026

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes
08:27

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

5.0K
Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
10:16

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles

Published on: August 17, 2022

3.8K
A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

3.0K

Area of Science:

  • Biotechnology
  • Immunology
  • Vaccinology

Background:

  • Deoxyribonucleic acid (DNA) vaccines are regaining prominence due to advances in synthetic biology and delivery systems.
  • Historically less potent, recent innovations have significantly boosted DNA vaccine immunogenicity, safety, and precision.

Purpose of the Study:

  • To review the engineering foundations, emerging applications, and innovations in DNA vaccine technology.
  • To evaluate the comparative performance and future role of DNA vaccines in global health strategies.
  • To propose a roadmap for integrating DNA vaccines into next-generation precision immunotherapy.

Main Methods:

  • Exploration of synthetic gene circuits, self-amplifying DNA (saDNA), and DNA-encoded monoclonal antibodies (DMAbs).
  • Review of novel adjuvants, electroporation techniques, and formulation strategies (lyophilization, encapsulation).
  • Analysis of clinical applications in infectious diseases, cancer immunotherapy, and immune-mediated conditions.

Main Results:

  • DNA vaccines now demonstrate enhanced immunogenicity, safety, and precision.
  • Key innovations enable programmable expression and robust immune activation.
  • DNA vaccines offer advantages in thermal stability, manufacturing, and storage compared to mRNA vaccines.

Conclusions:

  • DNA vaccine technology is a versatile and scalable platform with expanding clinical applications.
  • Interdisciplinary innovations are broadening the potential of DNA vaccines.
  • DNA vaccines are poised to become a significant component of future precision immunotherapy and global vaccine strategies.