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Evaluation of micro serum separator tubes for obtaining serum specimens suitable for serologic testing
Laboratory Animal Science
|December 1, 1985
Summary
Blood collection tubes did not affect Mycoplasma pulmonis or Sendai virus detection. Optical density values were similar between micro serum separator tubes and conventional blood collection tubes for these assays.
Area of Science:
- Veterinary Medicine
- Immunology
- Laboratory Science
Background:
- Accurate detection of pathogens like Mycoplasma pulmonis and Sendai virus is crucial in rodent research.
- Different blood collection methods may impact diagnostic assay sensitivity.
- Evaluating blood collection systems ensures reliable experimental outcomes.
Purpose of the Study:
- To compare the efficacy of micro serum separator tubes versus conventional 2 ml blood collection tubes for Mycoplasma pulmonis and Sendai virus detection.
- To determine if blood collection tube type influences diagnostic assay results.
Main Methods:
- Paired blood samples were collected from Sprague Dawley rats using two different tube types: micro serum separator tubes and conventional 2 ml tubes.
- Commercially available Enzyme-Linked Immunosorbent Assay (ELISA) kits were used to test for Mycoplasma pulmonis and Sendai virus.
- Optical density (OD) values, indicative of pathogen presence, were measured and compared between the two collection systems.
Main Results:
- No statistically significant differences were observed in optical density values between the micro serum separator tubes and the conventional 2 ml blood collection tubes.
- Both collection systems yielded comparable results for the detection of Mycoplasma pulmonis and Sendai virus.
Conclusions:
- Micro serum separator tubes are a viable alternative to conventional 2 ml blood collection tubes for Mycoplasma pulmonis and Sendai virus detection in Sprague Dawley rats.
- The choice of blood collection tube does not appear to compromise the accuracy of these specific ELISA-based diagnostic assays.