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Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

Rolling Circle Amplification Boosts Ultrasensitive Liquid Biopsy.

Jiaen You^1,2,3, Yingying Tan^1,2,3, Defu Kong⁴

¹Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, P. R. China.

|September 13, 2025

Summary

Rolling circle amplification (RCA) enhances liquid biopsy sensitivity and specificity. This review explores RCA

Keywords:

biomarker biosensor liquid biopsy rolling circle amplification

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Area of Science:

Biochemistry
Molecular Biology
Medical Diagnostics

Background:

Liquid biopsy offers minimally invasive disease diagnostics but requires improved sensitivity and specificity.
Current liquid biopsy methods face challenges due to complex biological sample matrices.

Purpose of the Study:

To review the principles and applications of Rolling Circle Amplification (RCA) in liquid biopsy.
To explore RCA-based biosensors for enhanced disease detection and monitoring.
To address clinical application prospects and challenges of RCA in liquid biopsy.

Main Methods:

Review of fundamental principles and classifications of Rolling Circle Amplification (RCA).
Systematic summary of RCA coupling strategies with various detection technologies.
Exploration of recent developments in RCA-based biosensors for liquid biopsy.

Main Results:

RCA enables rapid production of ultra-long DNA molecules for signal amplification.
Custom-designed RCA templates achieve high specificity and sensitivity in liquid biopsy.
Integration of RCA with detection technologies shows promise for clinical applications.

Conclusions:

RCA significantly enhances the sensitivity and specificity of liquid biopsy.
RCA-based biosensors represent a promising advancement for disease diagnostics.
Further research is needed to overcome challenges for widespread clinical integration of RCA in liquid biopsy.