Related Experiment Video

Updated: Jul 14, 2026

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates
09:12

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

16.6K

Potential modulation of microglial ferroptosis by Linggui Zhugan decoction in Alzheimer's disease through IL-17 and

Peng Tang1, Danjie Zhao2, Haixia Lin2

  • 1Department of Pharmacy, Chengdu Integrated TCM & Western Medicine Hospital, Chengdu 610000, China.

International Immunopharmacology
|September 13, 2025
PubMed

Insights

Traditional Chinese Medicine

Area of Science:

  • Neuroscience
  • Immunology
  • Pharmacology

Background:

  • Alzheimer's Disease (AD) involves cognitive decline and ferroptosis.
  • Microglial ferroptosis is implicated in AD pathogenesis.
  • Interleukin-17 (IL-17) and Tumor Necrosis Factor (TNF) signaling pathways are relevant.

Purpose of the Study:

  • To investigate the effects of Linggui Zhugan Decoction (LZD) on microglial ferroptosis in AD.
  • To explore the role of IL-17 and TNF signaling pathways in LZD's modulation of ferroptosis.
  • To assess LZD's therapeutic potential in an AD mouse model.

Main Methods:

  • Single-cell RNA sequencing in AD mice.
  • Network pharmacology analysis.
  • In vitro experiments with Aβ1-42-treated microglia.
  • In vivo behavioral tests and ferroptosis marker analysis.

Main Results:

  • LZD components potentially modulate ferroptosis-related pathways.
  • LZD treatment reduced IL-17 and TNF levels in microglia.
  • In vivo administration of LZD improved behavioral outcomes and modulated ferroptosis markers.
  • Alterations in microglial populations and ferroptosis gene expression were observed in AD mice.

Conclusions:

  • LZD shows potential in attenuating ferroptosis-associated pathology in AD.
  • Modulation of inflammatory pathways (IL-17, TNF) may underlie LZD's effects.
  • Further research is needed to confirm causal mechanisms of LZD in AD treatment.
Keywords:
Alzheimer's diseaseFerroptosisIL-17 pathwayNetwork pharmacologySingle-cell sequencingTNF pathway

More Related Videos

Assessing Microglial Phagocytosis of Myelin Debris in vitro Under Repeated Magnetic Stimulation08:34

Assessing Microglial Phagocytosis of Myelin Debris in vitro Under Repeated Magnetic Stimulation

Published on: June 17, 2025

926
Network Pharmacology and Validation of the Antidepressant Mechanisms of Qiangzhifang in a Chronic Restraint Stress-induced Depression Rat Model08:15

Network Pharmacology and Validation of the Antidepressant Mechanisms of Qiangzhifang in a Chronic Restraint Stress-induced Depression Rat Model

Published on: June 6, 2025

527

Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...