Hydraulic regimes-driven microbial community assembly and network stability in drinking water distribution systems:...

Hydraulic regimes within drinking water distribution system (DWDS) critically alter water quality and microbial communities, posing significant public health risks. However, how hydraulic regimes modify microbial (bacteria and fungi) assembly and network stability in DWDS remains poorly understood. This study investigated spatiotemporal dynamics of water quality and microbial succession across a full-scale DWDS, focusing on hydraulic regimes (transport distance (1-5 km) and stagnation) effects. Results indicated that increasing transport distance and hydraulic stagnation led to water quality deterioration, while concurrently amplifying microbial diversity and triggering community structural reorganization. Transport distance predominantly influenced microbial diversity during summer, whereas stagnation dominated community succession in winter. Besides, microbial species richness was greater in the 5 km tap water in summer, while higher species evenness was observed in winter. Integrated neutral community modeling and null model analysis (βNTI and RCbray metrics) demonstrated that a deterministic to stochastic transition in microbial assembly mechanisms - from niche-based selection at treatment plants to increasing stochastic dominance along distribution networks (|βNTI| < 2). Hydraulic regimes enhanced microbial stability and complexity, evidenced by elevated graph density and natural connectivity, conferring rapid resilience to bacterial and fungal communities. These findings establish a hydraulic-microbial ecology coupling framework within DWDS, proposing operational strategies for hydraulic regime optimization management in drinking water management.