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Updated: Jul 30, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
Structural characterization and immunomodulatory activity of polysaccharides from the roots of Artemisia argyi
Gai-Fan Ma1, Bin-Bin Zhao2, Peili Tang3
1School of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China.
Abstract:
The root of Artemisia argyi is traditionally utilized as a potential therapeutic agent for hypoimmunity and polysaccharides are considered as key substances for its immune activity. In this study, two inulin neoseries-type fructans (AARP-1 and AARP-2) were isolated from the root of A. argyi. The findings revealed that the molecular weights of AARP-1 and AARP-2 were 5.46 kDa and 3.21 kDa, respectively. Both polysaccharides predominantly consisted of fructose and exhibited specific backbone linkages including 2-β-D-Fruf, 1-α-D-Glcp, and 1,2-β-D-Fruf-. In addition, AARP-1 and AARP-2 could markedly promote the proliferation and phagocytic activity, as well as cytokine secretion in RAW264.7 cells via TLR4/MAPK signaling activation. Both polysaccharides alleviated cyclophosphamide-induced immunosuppression in mice by reversing weight loss, leukopenia, clearance index and immune organ index. These results offer valuable insights into the structural properties and immunological roles of AARP-1 and AARP-2, emphasizing their potential as promising natural compounds for immunotherapeutic and related biomedical applications.
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