Related Experiment Video
Updated: Jan 17, 2026
In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
Published on: September 28, 2017
Pineal Gland and Melatonin are Associated with Serum Element Metabolism in Rats
Aylin Üstün1, Zeynep Köykun2, Haluk Gümüş3
1Department of Medical Services and Techniques, Vocational School of Health Services, Konya, Türkiye.
Introduction:
The aim of this study is to investigate the relationship of melatonin supplementation with serum element metabolism in pinealectomized rats.
Methods:
The research was conducted on 32 adult male Spraque-Dawley rats. The study protocol was approved by the local animal ethics committee.Animals were divided into four equal groups. Control (Group 1), Melatonin (Group 2), Pinealectomy "Px" (Group 3), Pinealectomy+melatonin (Group 4). Animals in groups 2 and 4 were given intraperitoneal (ip) melatonin support (4 weeks/day; 3 mg/kg melatonin). Animals in groups 3 and 4 underwent pinealectomy under general anesthesia. At the end of the applications, serum element levels were determined by atomic emission (μg/dl) in the blood samples taken from the sacrificed animals.
Results:
While pinealectomy increased chromium and manganese levels in Group 3 (p<0.001), it caused significant suppression of magnesium, calcium and zinc levels (p<0.001). Melatonin supplementation in pinealectomized animals (Group 4) treated the impairments in the mentioned parameters.
Conclusion:
The results of the current study show that the melatonin hormone secreted from the pineal gland has a regulatory effect on serum element metabolism. This study is the first to examine the relationship between the pineal gland and element metabolism as a whole.
More Related Videos
07:04A High-performance Liquid Chromatography Measurement of Kynurenine and Kynurenic Acid: Relating Biochemistry to Cognition and Sleep in Rats
Published on: August 19, 2018
06:53Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
Published on: November 11, 2016
Related Concept Videos
01:02The Pineal Gland
The primary secretion of the pineal gland is the hormone melatonin, derived from serotonin. The concentration of melatonin in the...
01:27Major Hormones and Their Functions
Oxytocin, produced in the hypothalamus and released by the pituitary gland, plays a role in social bonding, childbirth, and...
02:19Circadian Rhythms and Gene Regulation
01:19Management of Insomnia
01:19Factors Affecting Drug Biotransformation: Biological
Species differences: Variations in enzyme systems across species can cause disparities in drug metabolism. For instance, humans may metabolize certain drugs faster than rodents, altering therapeutic effects.
Strain differences: Genetic variations within a species can result in differing enzyme activity, impacting drug response and toxicity. For example, some mouse strains may...