Irisin/FNDC5 Regulates Endothelial Function to Improve Post-Stroke-Induced Cognitive Dysfunction by Stimulating...

Area of Science:

• Neuroscience
• Molecular Biology
• Exercise Physiology

Background:

• Post-stroke cognitive impairment (PSCI) significantly impacts patient quality of life and survival.
• While exercise is beneficial, the underlying mechanisms for preventing and treating PSCI are not fully understood.

Purpose of the Study:

• To investigate the therapeutic effects of irisin on cognitive deficits in a mouse model of PSCI.
• To elucidate the molecular mechanisms, including signaling pathways and molecular markers, involved in irisin's action.

Main Methods:

• Mice with PSCI were treated with irisin.
• Behavioral tests assessed learning and memory.
• Hippocampal expression levels of PECAM-1, GFAP, VEGF, and the AMPK-eNOS pathway were measured.

Main Results:

• Irisin treatment significantly improved learning and memory in PSCI mice.
• This was associated with reduced cerebral infarction size and neuronal death.
• Irisin activated the AMPK-eNOS pathway, increased VEGF expression, promoted brain angiogenesis, and reduced astrocytic scarring.

Conclusions:

• Irisin may mediate the cognitive benefits of exercise in PSCI.
• Irisin demonstrates potential as a therapeutic agent for PSCI by improving endothelial function and reducing neuroinflammation.