Efficient Expression and Purification of Recombinant Mouse Dimeric IgA
Antonia Geisse1, Tao Zhang2,3, Jonathan Schreiber1
1Institute of Molecular Medicine and Experimental Immunology, University Hospital Bonn, Bonn, Germany.
European Journal of Immunology
|September 15, 2025
Summary
Researchers developed a new method to produce and purify dimeric immunoglobulin A (IgA) from single B cells. This technique enables the study of mucosal antibody responses at the single-cell level.
Area of Science:
- Immunology
- Molecular Biology
Background:
- Immunoglobulin A (IgA) is the primary antibody on mucosal surfaces.
- IgA predominantly exists as a dimer in mucosal secretions.
- Understanding mucosal immunity requires studying IgA at a single-cell level.
Purpose of the Study:
- To develop an efficient method for producing and purifying monoclonal mouse dimeric IgA.
- To enable the study of mucosal antibody responses at the single-cell level.
Main Methods:
- Production and purification of monoclonal mouse dimeric IgA.
- Utilizing single B cell immunoglobulin (Ig) transcripts.
Main Results:
- An easy, scalable, and efficient method was established.
- The method is broadly applicable for dimeric IgA production and purification.
Conclusions:
- The developed method facilitates the investigation of mucosal antibody responses.
- This technique supports single-cell level analysis of dimeric IgA.
